Analysis of Plasma-Derived Exosomal MicroRNAs as Potential Biomarkers for Canine Idiopathic Epilepsy

This study investigates the role of plasma-derived exosomal microRNAs (miRNAs) as diagnostic and prognostic biomarkers in canine idiopathic epilepsy, one of the most prevalent neurological disorders in both dogs and humans. Exosomes were isolated from the plasma of 23 dogs divided into three groups: drug-responsive epilepsy, drug-resistant epilepsy, and healthy controls. Exosome characterization was performed using transmission electron microscopy, nanoparticle tracking analysis, and dot blotting. Seven specific miRNAs were quantified via qPCR. The results revealed significant differences in miRNA levels, including miR-16, miR-93-5p, and miR-142, between the groups, identifying distinct patterns for drug-sensitive and drug-resistant epileptic dogs. These findings highlight plasma exosomes as a valuable source of biomarkers and demonstrate the potential of miRNAs for the diagnosis and prognosis of canine idiopathic epilepsy.